Catalyst® Progesterone for in-house measurement of progesterone in plasma from bitches

canine progesterone

This article is sponsored content brought to you by IDEXX.

Progesterone is a female reproductive hormone. Measurement of progesterone in blood plasma (plasma) or serum from the bitch is an important element of:

  • Predicting and confirming ovulation to determine optimal breeding time and maximise fertility.1
  • Predicting parturition.2
  • Investigating reproductive abnormalities.3

Catalyst® Progesterone is a new immunoassay from IDEXX that is designed to provide prompt and reliable in-clinic measurement of progesterone in canine samples. It works with both the IDEXX Catalyst One® and IDEXX Catalyst Dx® chemistry analysers. It has a reportable range of 0.6–63.6 nmol/L. In clinical practice, various methods have been used to monitor progesterone in the bitch. For many decades, radioimmunoassay*(RIA) was regarded as a gold standard; however, the originally validated assay was discontinued in 2014,4 and liquid chromatography-mass spectrometry (LC-MS) has been proposed as the gold standard.5 Because LC-MS is not typically available in veterinary reference laboratories, chemiluminescent immunoassay (CLIA) on IMMULITE® analysers† is more widely used.‡ However, despite strong correlation between CLIA and reference methods,6 clinically significant bias between methods has also been demonstrated.4 The situation is further complicated by differing performance between iterations of the CLIA methodology.7

canine progesterone

For progesterone assays used with canine samples, it is important to have accuracy and precision in the range associated with ovulation, which is 9.5-31.2 nmol/L or lower.4  

Catalyst® Progesterone demonstrates strong correlation (r = 0.98; r = 0.99) to the reference method of LC-MS and precision in the range of clinical interest. Catalyst® Progesterone produces accurate and precise results when used to quantify progesterone in plasma samples from bitches. This new immunoassay provides a reliable and convenient option to measure canine progesterone in-house.

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References: 1. Concannon P, Hansel W, McEntee K. Changes in LH, progesterone and sexual behavior associated with preovulatory luteinization in the bitch. Biol Reprod. 1977;17(4):604–613. 2. De Cramer KGM, Nöthling JO. The precision of peri-oestrous predictors of the date of onset of parturition in the bitch. Theriogenology. 2017;96:153–157. 3. Meyers-Wallen VN. Unusual and abnormal canine estrous cycles. Theriogenology. 2007;68(9):1205–1210. 4. Nöthling JO, De Cramer KGM. Comparing the values of progesterone in the blood of bitches as measured with a chemiluminescence immunoassay and a radioimmunoassay. Reprod Dom Anim. 2018;53(5):1136–1141. 5. Stanczyk FZ, Lee JS, Santen RJ. Standardization of steroid hormone assays: why, how, and when? Cancer Epidemiol Biomarkers Prev. 2007;16(9):1713–1719. 6. Kutzler MA, Mohammed HO, Lamb SV, Meyers-Wallen VN. Accuracy of canine parturition date prediction from the initial rise in preovulatory progesterone concentration. Theriogenology. 2003;60(6):1187–1196. 7. Schmicke M, Urhausen C, Wolf K, Schmidt S, Günzel Apel AR. Evaluation of the blood progesterone concentration in the bitch measured by chemiluminescence immunoassay at the day of ovulation. Tierärztl Prax Ausg K Kleintiere Heimtiere. 2016;44(5):317–322. 8. Zady MF. Z-12: Correlation and simple least squares regression Westgard QC website. www.westgard.com/lesson42.htm. Accessed January 15, 2019. * 125-I radioimmunoassay (Coat-A-Count® radioimmunoassay; Siemens Health Care Diagnostics Inc., Los Angeles, California, USA) †Siemens Medical Solutions Diagnostics, Los Angeles, California, USA. ‡CLIA is used for canine samples at IDEXX Reference Laboratories.


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